The Safe and Effective Plateletpheresis

At the Research and Production Center of Transfusion platelet production have mainly supported (about 99%) by apheresis. However, apheresis donations have limitations caused by donor fitness due to both their preferences and their platelet pre-count validity. So, it is important to maintain of single donor high-dose plateletpheresis by improving its efficacy and safety. The aim of study was to develop of safe-effective approaches of plateletpheresis. Donors had been attracted to the study if they had filed of informed consent for post-collection blood sampling in advance. They were selected with following criteria: male, age 18-60 years, weight ≥ 55 kg, Hb ≥ 125.0 g/L, PLTs ≥ 160.0 × 109/L. Target number of platelets need to be obtained was chosen under calculation of estimated donor post-collection platelets. Platelets were harvested by «Hemonetics MCS plus» separators with LDP protocol, blood samples were counted by «Sysmex» hematology analyzer. Thus, 16 apheresis procedures with volunteer-donors were studied. The following results are demonstrated in the present study: donor pre-collection platelets 286.0 × 109/L ± 27.2; donor blood volume calculated by separator 5481.6 mL ± 408.5; platelet yield 473.1 × 109/unit ± 47.7; donor blood processed 3190.8 mL ± 189.7; donor platelets processed 763.0 × 109 ± 55.6; actual donor post-collection platelets measured by lab 193.3 × 109/L ± 18.6; platelet collection efficacy 61.9% ± 3.0. The values of estimated postcollection platelets 199.0 × 109/L ± 21.3 were not significantly different from those with actual post-collection platelets 193.3 × 109/L ± 18.6 (χ2=0.401). Thus, plateletpheresis efficacy has to be controlled using calculation of estimated donor post-collection platelets by making sure that safe threshold of post-apheresis platelet number is provided. The further studies have to be proceeding due to small number of observations.